Purity¶
Definition¶
Purity is the quantitative measure of the target peptide relative to all components in a sample, typically expressed as a percentage. It is a critical quality attribute for research peptides.
Overview¶
Peptide purity is most commonly determined by HPLC analysis using area normalization, where the area of the main peptide peak is divided by the total area of all detected peaks. Purity specifications (e.g., ≥98%, ≥99%) indicate the minimum acceptable purity level.
Technical Explanation¶
Purity vs. Identity: - Purity: Measures how much of the material is the desired peptide - Identity: Confirms that the peptide is the correct one (via MS, sequence analysis) - Both are required for complete quality assessment
How Purity Is Measured: - HPLC with UV detection (most common) - Area normalization: Main peak area / Total peak area × 100% - Impurities appear as additional peaks at different retention times
Common Reporting Methods: - Area % (HPLC area normalization) - Weight % (corrected for counter-ion and water content) - Anhydrous, solvent-free basis
Interpretation Considerations: - Purity alone does not indicate identity - A single sharp peak could be the wrong peptide - Always verify purity AND identity together
Importance in Peptide Documentation¶
Purity is the most frequently cited specification on product listings, COAs, and TDS documents. It directly impacts research reproducibility and is often the first attribute researchers evaluate when selecting a supplier.
Related Terms¶
Frequently Asked Questions¶
Q: Is 98% purity always sufficient for research? A: For most in vitro research, ≥98% is standard. For sensitive in vivo work, higher purity (≥99%) may be preferred. Very low-purity material (<95%) may yield unreliable results.
Q: Does purity include water and salt content? A: HPLC area % purity reports only the relative proportions of peptide-related species. Water content, residual solvents, and counter-ion content are measured separately (by LOD, KF, or TGA) and reported on the COA.