Semaglutide — Technical Data Sheet¶

1. General Product Information¶

Field	Details
Product Name	Semaglutide
CAS Number	910463-68-2
Molecular Formula	C187H291N45O59
Molecular Weight	4113.58 Da
IUPAC Name	N-[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-6-amino-1-[[(2S)-1-[(2S)-2-[[(2S)-4-carboxy-1-[[(2S)-1-[[(2S)-5-carbamimidamido-1-[[(2S)-1-[[(2S)-1-[[(2S)-5-carbamimidamido-1-[[(2S)-1-[[(2S)-4-carboxy-1-[[(2R)-1-[[(2S)-1-[[(2S)-5-carbamimidamido-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-5-carbamimidamido-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2R)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-5-carbamimidamido-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-hexadecanoylpyrrolidine-2-carbonyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-methylpentanoyl]amino]propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-4-methylpentanoyl]amino]propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-4-carboxybutanoyl]amino]propanoyl]amino]-4-methylpentanoyl]amino]-4-carboxybutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-methylbutanoyl]amino]-4-carboxybutanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]acetyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-methylbutanoyl]amino]propanoyl]amino]-4-carboxybutanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]acetyl]pyrrolidine-2-carbonyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-4-methylpentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-4-methylpentanoyl]amino]propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-4-carboxybutanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-4-methylpentanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]propanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-methylbutanoyl]amino]-4-carboxybutanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-4-carboxybutanoyl]amino]-3-hydroxypropanoyl]amino]-5-carbamimidamidopentanoyl]amino]-4-carboxybutanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-methylpentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-4-methylpentanoyl]amino]propanoyl]amino]-4-carboxybutanoyl]amino]-4-carboxybutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-4-methylpentanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-4-carboxybutanoyl]amino]-4-carboxybutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-4-methylpentanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-4-methylpentanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-3-(1H-imidazol-4-yl)propanoyl]amino]-3-methylbutanoyl]amino]

Note: The full IUPAC name is provided for chemical reference. For routine laboratory use, refer to the product by its common name Semaglutide or CAS number 910463-68-2.

2. Product Specifications¶

Parameter	Specification
Peptide Content	≥ 80% peptide content (by mass)
Purity (HPLC)	≥ 99%
Appearance	White to off-white lyophilized powder
Salt Form	Acetate or TFA salt (batch dependent)
Solubility	Soluble in water, PBS, and saline at > 5 mg/mL
pH (1% solution in water)	4.0 – 7.0
Water Content (KF)	≤ 8.0%
Endotoxin (LAL)	≤ 1.0 EU/mg
Molecular Weight (LC-MS)	4113.58 ± 1 Da
Sequence Confirmation	Confirmed by MS/MS

3. Sequence Information¶

Property	Value
Molecule Type	Linear peptide
Number of Amino Acids	31
Amino Acid Sequence	H-His-Aib-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu-Gly-Gln-Ala-Ala-Lys-Glu-Phe-Ile-Ala-Trp-Leu-Val-Arg-Gly-Arg-Gly-OH
Modifications	C-18 fatty diacid (hexadecanedioic acid) conjugated to Lys at position 20 via a hydrophilic linker (gamma-Glu-2xOEG)
Disulfide Bonds	None
Secondary Structure	Partial alpha-helix in solution

4. Activity Profile¶

Property	Value
Target	GLP-1 Receptor
Receptor Type	Class B G protein-coupled receptor (GPCR)
Mechanism	Full agonist
Half-life	Approximately 165 hours (7 days) in human plasma
Binding Affinity (IC50)	~ 0.5 nM for human GLP-1 receptor
cAMP Potency (EC50)	~ 0.5 – 1.5 nM

5. Quality Assurance¶

RPL Peptide ensures the following quality control measures for all Semaglutide batches:

Purity Analysis: Reverse-phase HPLC with UV detection at 214 nm and 280 nm
Mass Confirmation: LC-MS for intact molecular weight determination
Sequence Confirmation: MS/MS fragmentation analysis
Water Content: Karl Fischer titration
Endotoxin Testing: LAL gel-clot method
Appearance: Visual inspection
Residual TFA: Ion chromatography (where applicable)
Peptide Content: Amino acid analysis or UV quantitation

Each batch is accompanied by a Certificate of Analysis (COA) detailing all measured parameters.

6. Reconstitution Protocol¶

Step	Action
1	Equilibrate the vial to room temperature for 15 minutes
2	Gently tap the vial to ensure all powder settles at the bottom
3	Remove flip-off cap and disinfect the rubber septum with 70% isopropanol
4	Slowly inject sterile water for injection, PBS, or sterile saline along the vial wall
5	Swirl gently — do not vortex — until completely dissolved
6	If needed, further dilute in appropriate buffer for the specific assay

Reconstitution Volume Table¶

Bottle Size	Recommended Reconstitution Volume
10 mg	1.0 – 2.0 mL
15 mg	1.5 – 3.0 mL
20 mg	2.0 – 4.0 mL
30 mg	3.0 – 6.0 mL
40 mg	4.0 – 8.0 mL
50 mg	5.0 – 10.0 mL
60 mg	6.0 – 12.0 mL
100 mg	10.0 – 20.0 mL
120 mg	12.0 – 24.0 mL

7. Storage Conditions¶

Condition	Specification
Lyophilized (Long-term)	-20 °C, desiccated, protected from light
Lyophilized (Short-term)	2 – 8 °C for up to 4 weeks
Reconstituted	2 – 8 °C for up to 7 days
Aliquoted and Frozen	-20 °C for up to 4 weeks (single-use aliquots)
Transport	Cold chain (dry ice or gel packs)

8. Regulatory & Safety Classification¶

Classification	Status
For Human Use	No — laboratory research use only
For Veterinary Use	No
Hazard Classification	Not classified as hazardous under GHS
UN Number	Not applicable
Transport Classification	Not regulated as dangerous goods

9. Disclaimer¶

FOR LABORATORY RESEARCH USE ONLY. NOT FOR HUMAN OR VETERINARY USE.

This technical data sheet is provided for informational purposes only. RPL Peptide makes no warranties, express or implied, regarding the accuracy, completeness, or suitability of the information provided. The user assumes all risk and liability associated with the use of this product. It is the responsibility of the user to ensure that the product is used in compliance with all applicable laws, regulations, and institutional guidelines.

Official Technical Documentation

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