TB-500 Technical Manual — Chapter 1: Technical Data Sheet¶

Field	Value
Document ID	RPL-TM-TB5-001
Chapter	1 of 18
Title	Technical Data Sheet
Version	1.0
Effective Date	July 2026

1.1 Product Overview¶

TB-500 is a synthetic peptide fragment corresponding to the active actin-binding domain (LKKTETQ) of Thymosin Beta-4 (Tβ4), a naturally occurring 43-amino-acid polypeptide. Tβ4 is the major actin-sequestering molecule in eukaryotic cells and plays a central role in cytoskeletal organization, cell migration, angiogenesis, and tissue repair. TB-500 (Tβ4 fragment) retains the core biological activity of full-length Tβ4, specifically the LKKTETQ motif responsible for binding to monomeric G-actin and modulating actin polymerization.

1.2 General Information¶

Property	Specification
Product Name	TB-500 (Thymosin Beta-4 Fragment)
CAS Number	77591-33-4
Molecular Formula	C₃₆H₆₆N₈O₁₂ (calculated from LKKTETQ sequence)
Molecular Weight	2,163 Da (2,163.5 g/mol)
Sequence	LKKTETQ (7 amino acids)
IUPAC Name	L-Leucyl-L-lysyl-L-lysyl-L-threonyl-L-α-glutamyl-L-threonyl-L-glutamine
Product Type	Synthetic peptide fragment
Synonyms	Tβ4 fragment, Thymosin Beta-4 (active fragment), LKKTETQ peptide, TB4 fragment
Appearance	White lyophilized powder
Purity (HPLC)	≥99%

1.3 Quality Assurance¶

Parameter	Method	Specification
Purity	HPLC (214 nm)	≥99.0%
Peptide Content	Amino Acid Analysis	≥80.0%
Molecular Weight Confirmation	LC-MS (ESI+)	Consistent with theoretical mass
Counter-ion	TFA⁻ (Trifluoroacetate)	<5.0% by weight
Water Content (KF)	Karl Fischer	<6.0%
Endotoxin Level	LAL Test	<1.0 EU/mg
Appearance	Visual Inspection	White lyophilized powder
Solubility	Visual Assessment	Clear, colorless solution in water/PBS/saline

1.4 Reconstitution Information¶

Parameter	Recommendation
Reconstitution Solvent	Sterile water for injection (WFI), sterile PBS (pH 7.4), or sterile 0.9% saline
Concentration Range	1–10 mg/mL
Recommended Stock Concentration	5 mg/mL
Mixing	Gently swirl; avoid vigorous vortexing
Post-Reconstitution Storage	2–8°C for up to 14 days; -20°C for extended storage
Freeze-Thaw Cycles	Minimize; aliquot for single-use to avoid repeated freeze-thaw

1.5 Packaging and Storage¶

Parameter	Specification
Package Sizes	2 mg, 5 mg, 10 mg per vial
Packaging Configuration	10 vials per box
Vial Type	Clear glass vial, 2 mL / 5 mL / 10 mL capacity
Closure	Rubber stopper with aluminum flip-off seal
Labeling	Product name, batch number, quantity, purity, storage condition, date of manufacture, expiry date
Storage Condition	2–8°C (refrigerated), sealed, protected from light and moisture
Shelf Life	24 months from date of synthesis when stored as specified
Transport	Cold chain shipping (2–8°C) recommended; short-term ambient transport (<72 h) acceptable

1.6 Ordering Information¶

Package Size	Catalog Reference	Price Range (USD) per Box
2 mg × 10 vials	RPL-TB5-2MG	$89
5 mg × 10 vials	RPL-TB5-5MG	$129
10 mg × 10 vials	RPL-TB5-10MG	$158

Disclaimer

This document is provided for informational and reference purposes only. TB-500 (Thymosin Beta-4 Fragment, CAS: 77591-33-4) is intended for laboratory research use only. It is not for human consumption, not for medical use, not for veterinary use, and not for food or cosmetic applications. The information herein is based on current scientific knowledge and regulatory guidelines. RPL Peptide makes no warranties, express or implied, regarding the accuracy, completeness, or suitability of this information for any particular purpose. Users are responsible for ensuring compliance with all applicable laws, regulations, and institutional guidelines governing the use of research peptides. RPL Peptide shall not be held liable for any damages arising from the use or misuse of this product or the information contained in this document.

Document Control

Version	Date	Author	Change Description
1.0	July 2026	RPL Peptide Technical Documentation Team	Initial release

End of Chapter 1