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RPL Peptide

Official Technical Documentation

Mass Spectra Library


Mass Spectrometry Analysis — Tirzepatide

Analytical Method

Parameter Specification
Method Liquid Chromatography-Mass Spectrometry (LC-MS)
Ionization Electrospray Ionization (ESI), Positive Mode
Mass Analyzer Quadrupole Time-of-Flight (Q-TOF)
Scan Range m/z 500–2500
Resolution ≥20,000 (FWHM)
Mass Accuracy ±0.5 Da

Expected Mass Data

Parameter Value
Molecular Formula C₂₂₅H₃₄₈N₄₈O₆₈
Calculated Average Mass ~4,813 Da
Monoisotopic Mass [M+H]⁺ = ~4,814 Da
Charge State Series [M+3H]³⁺, [M+4H]⁴⁺, [M+5H]⁵⁺, [M+6H]⁶⁺

Acceptance Criteria

Parameter Limit
Mass Accuracy ±0.5 Da of calculated value
Signal-to-Noise ≥10:1 for base peak

Representative Mass Spectrum

[Mass spectrum image would be inserted here for each batch]

Typical Charge State Distribution

Charge State m/z (Calculated) m/z (Observed)
[M+3H]³⁺
[M+4H]⁴⁺
[M+5H]⁵⁺
[M+6H]⁶⁺
[M+7H]⁷⁺

Batch-Specific Data

Batch Number Observed Mass (Da) Mass Accuracy (Da) Status
[Batch-001]

Notes

  • Mass spectrometry confirms peptide identity and molecular integrity
  • Full mass spectra archived and available upon request
  • Deconvolution performed using MaxEnt or similar algorithm

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Official Technical Documentation

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